thr77 login Options
thr77 login Options
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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in several tumors, which results in chromosome instability, and therefore may lead to tumorigenesis. This protein was uncovered to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a important purpose in marketing cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two more splice variants have been documented but specific nucleotide sequence information isn't however offered.
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Blocking peptides are peptides that bind specially on the target antibody and block antibody binding. These peptide generally consists of the epitope regarded with the antibody.
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This manual outlines even more achievable optimization of mobile and immunoassay parameters to ensure the best possible success are...
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This guidebook outlines further possible optimization of mobile and immunoassay parameters to ensure the best possible outcomes are...
Antibodies certain to the blocking peptide not bind towards the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is particular.
Target Info Cyclin E belongs for the very conserved cyclin loved ones, whose associates are characterized by a dramatic periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins get more info show unique expression and degradation patterns which add on the temporal coordination of each mitotic celebration. Cyclin E forms a posh with and features for a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates within the G1-S stage boundary and is particularly degraded as cells progress through S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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